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It is postulated that the volume reduction of the umbilical cord blood unit in the dextran or albumin before its infusion into the patients will result in improved cell variability.  To be able to get the best stem cells, it is always best to take them from the umbilical cord blood.  They are then stored in a cord blood bank for the best collection of cord blood.  This is based on vitro study which showed that stem cell viability can be improved by the volume reducing umbilical cord blood units before it is infused to restore the suspension’s osmolarity.

It was also suggested that cord blood collection can protect the stem cells against severe osmotic stress that is associated with the infusion of the suspended cells in medium with high dimethyl-sulfoxide concentrations.

The Neutrophils were the major cell population that is affected by the in vitro incubation whereas the mononuclear cells which include the pluripotent stem cells were resistant relatively to the in vitro toxic effects of the dimethyl-sulfoxide.

If you will reduce both the cell lysis products and dimethyl-sulfoxide volumes, washing may decrease also the adverse reactions that are associated with the cryo preserved units infusion.  The volume reducing grafts after thawing, however, can reduce the hematopoietic stem cells numbers which is infused into the patient because of cell loss that happens during manipulation.  Studies have shown that high nucleated cell dose infusion is good prognostic factor both for the engraftment and for the survival in the umbilical cord blood transplantation.

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